Journal: bioRxiv

Article Title: Decreased Colonic Activin Receptor-Like Kinase 1 Disrupts Epithelial Barrier integrity and is associated with a poor clinical outcome in Crohn’s disease

doi: 10.1101/2020.02.21.960070

Figure Lengend Snippet: (A) Proliferation- and stemness-related gene expression in colonic epithelial cells isolated from CD patients (N=15) and NIBD controls (N=12). Each gene expression was normalized to GAPDH . ( B ) Representative immunohistochemical images of OLFM4 expression in the colonic crypts of NIBD controls (left) and CD patients (right). The percentage of OLFM4 staining area in colonic crypts was compared between CD patients and NIBD controls (N=4 per group). *P<0.05, ***P<0.001. P-values were determined by Mann-Whitney test.

Article Snippet: Rabbit anti-human CA1 antibody, Rabbit anti-human OLFM4 antibody, and Mouse/Rabbit IgG VisUCyte HRP polymer antibody were purchased from Novus Biologicals (CO, USA), Cell Signaling Technology (MA, USA), and R&D Systems.

Techniques: Gene Expression, Isolation, Immunohistochemical staining, Expressing, Staining, MANN-WHITNEY